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iscript cdna synthesis kit bio rad 1708891  (Bio-Rad)


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    Bio-Rad iscript cdna synthesis kit bio rad 1708891
    Iscript Cdna Synthesis Kit Bio Rad 1708891, supplied by Bio-Rad, used in various techniques. Bioz Stars score: 99/100, based on 49689 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/iscript+cdna+synthesis+kit+(bio%E2%80%90rad%2C+1708891)/iScript+cDNA+Synthesis+Kit/pmc12728157-74-0-4
    Average 99 stars, based on 49689 article reviews
    iscript cdna synthesis kit bio rad 1708891 - by Bioz Stars, 2026-09
    99/100 stars

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    Related Articles

    Spectrophotometry:

    Article Title: Bisphenol-A mediated ubiquitinome alteration triggers PPAR-alpha ubiquitination, affecting trophoblast cell migration
    Article Snippet: .. The quantity and quality of the extracted RNA were assessed using a NanoDrop spectrophotometer (Thermo #ND-ONE-W). cDNA was synthesized with 1 μg of RNA using the iScript cDNA Synthesis Kit (Bio-Rad #1708891) in a 20 μL reaction containing iScript Reverse Transcriptase and 5x Reaction Mix. ..

    Synthesized:

    Article Title: Bisphenol-A mediated ubiquitinome alteration triggers PPAR-alpha ubiquitination, affecting trophoblast cell migration
    Article Snippet: .. The quantity and quality of the extracted RNA were assessed using a NanoDrop spectrophotometer (Thermo #ND-ONE-W). cDNA was synthesized with 1 μg of RNA using the iScript cDNA Synthesis Kit (Bio-Rad #1708891) in a 20 μL reaction containing iScript Reverse Transcriptase and 5x Reaction Mix. ..

    cDNA Synthesis:

    Article Title: Bisphenol-A mediated ubiquitinome alteration triggers PPAR-alpha ubiquitination, affecting trophoblast cell migration
    Article Snippet: .. The quantity and quality of the extracted RNA were assessed using a NanoDrop spectrophotometer (Thermo #ND-ONE-W). cDNA was synthesized with 1 μg of RNA using the iScript cDNA Synthesis Kit (Bio-Rad #1708891) in a 20 μL reaction containing iScript Reverse Transcriptase and 5x Reaction Mix. ..

    Article Title: Cobinamide, a Vitamin B12 Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation
    Article Snippet: Band intensity was determined by densitometric scanning using a Li-Cor Odyssey instrument over a range where band intensity was linear. .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific). .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific).

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System. ..

    Article Title: Biomimetic hybrid nanoparticles for dual regulation of macrophage pyroptosis and metabolism in rheumatoid arthritis.
    Article Snippet: The concentration and purity of total RNA were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher, USA). .. A total of 2 μg of qualified total RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, USA, 1708891). qRT-PCR was performed in a reaction system containing cDNA, SYBR Premix Ex Taq (TaKaRa, Japan, RR420A), and gene-specific forward/reverse primers using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher, USA). ..

    Article Title: GIPR:GCGR co-agonism restores normal weight in obese rodents
    Article Snippet: RNA from the liver lysate was extracted using RNeasy mini kit (Qiagen, Cat #74106). .. RNA was extracted using RNeasy lipid tissue mini kit (Qiagen, Cat #74804) or RNeasy Fibrous tissue mini kit (Qiagen, Cat #74704) in the case of muscle. cDNA was generated using iScript cDNA synthesis kit (Bio-Rad, Cat #1708891). .. Gene expression was performed with TaqMan probes and TaqMan gene expression master mix (Cat #4370074) from Applied Biosystems using QuantStudio 5 realtime PCR system (Applied Biosystems).

    Article Title: Generation of spinal cord organoids from human induced pluripotent stem cells caudalised to a lumbar fate.
    Article Snippet: Real-time qPCR for spinal cord region specification Five day 40 organoids were pooled per sample from each differentiation (3 independent differentiations) and RNA was extracted using the RNeasy Mini kit (Qiagen 74104) and RNaseFree DNase set (QIAGEN 79254) as per manufacturer’s protocol. .. For RT-PCR, cDNA was reverse transcribed from 500ng of RNA using the iScript cDNA Synthesis Kit (Bio-Rad 1708891). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) on a CFX96 machine (Bio-Rad). ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Article Title: Chronic early-life exposure to diisopentyl phthalate, a plasticizer induced aging and impaired innate immunity via insulin-like and p38 MAPK pathways in Caenorhabditis elegans.
    Article Snippet: Plasticizers are widely used industrial chemicals, in addition, their extensive application has raised growing concerns about potential exposure-related health risks.. Diisopentyl phthalate (DiPP), commonly used in the production of plastics and coatings, remains poorly characterized with respect to its toxicological effects.. In this study, Caenorhabditis elegans was employed as the research model to evaluate the impact of early long-time exposure to DiPP on aging and innate immunity.

    Reverse Transcription:

    Article Title: Bisphenol-A mediated ubiquitinome alteration triggers PPAR-alpha ubiquitination, affecting trophoblast cell migration
    Article Snippet: .. The quantity and quality of the extracted RNA were assessed using a NanoDrop spectrophotometer (Thermo #ND-ONE-W). cDNA was synthesized with 1 μg of RNA using the iScript cDNA Synthesis Kit (Bio-Rad #1708891) in a 20 μL reaction containing iScript Reverse Transcriptase and 5x Reaction Mix. ..

    Article Title: Cobinamide, a Vitamin B12 Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation
    Article Snippet: Band intensity was determined by densitometric scanning using a Li-Cor Odyssey instrument over a range where band intensity was linear. .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific). .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific).

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System. ..

    Article Title: Biomimetic hybrid nanoparticles for dual regulation of macrophage pyroptosis and metabolism in rheumatoid arthritis.
    Article Snippet: The concentration and purity of total RNA were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher, USA). .. A total of 2 μg of qualified total RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, USA, 1708891). qRT-PCR was performed in a reaction system containing cDNA, SYBR Premix Ex Taq (TaKaRa, Japan, RR420A), and gene-specific forward/reverse primers using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher, USA). ..

    Article Title: Generation of spinal cord organoids from human induced pluripotent stem cells caudalised to a lumbar fate.
    Article Snippet: Real-time qPCR for spinal cord region specification Five day 40 organoids were pooled per sample from each differentiation (3 independent differentiations) and RNA was extracted using the RNeasy Mini kit (Qiagen 74104) and RNaseFree DNase set (QIAGEN 79254) as per manufacturer’s protocol. .. For RT-PCR, cDNA was reverse transcribed from 500ng of RNA using the iScript cDNA Synthesis Kit (Bio-Rad 1708891). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) on a CFX96 machine (Bio-Rad). ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Isolation:

    Article Title: Cobinamide, a Vitamin B12 Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation
    Article Snippet: Band intensity was determined by densitometric scanning using a Li-Cor Odyssey instrument over a range where band intensity was linear. .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific). .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific).

    Article Title: Chronic early-life exposure to diisopentyl phthalate, a plasticizer induced aging and impaired innate immunity via insulin-like and p38 MAPK pathways in Caenorhabditis elegans.
    Article Snippet: Plasticizers are widely used industrial chemicals, in addition, their extensive application has raised growing concerns about potential exposure-related health risks.. Diisopentyl phthalate (DiPP), commonly used in the production of plastics and coatings, remains poorly characterized with respect to its toxicological effects.. In this study, Caenorhabditis elegans was employed as the research model to evaluate the impact of early long-time exposure to DiPP on aging and innate immunity.

    Polymerase Chain Reaction:

    Article Title: Cobinamide, a Vitamin B12 Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation
    Article Snippet: Band intensity was determined by densitometric scanning using a Li-Cor Odyssey instrument over a range where band intensity was linear. .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific). .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific).

    Real-time Polymerase Chain Reaction:

    Article Title: Cobinamide, a Vitamin B12 Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation
    Article Snippet: Band intensity was determined by densitometric scanning using a Li-Cor Odyssey instrument over a range where band intensity was linear. .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific). .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific).

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System. ..

    Article Title: Biomimetic hybrid nanoparticles for dual regulation of macrophage pyroptosis and metabolism in rheumatoid arthritis.
    Article Snippet: The concentration and purity of total RNA were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher, USA). .. A total of 2 μg of qualified total RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, USA, 1708891). qRT-PCR was performed in a reaction system containing cDNA, SYBR Premix Ex Taq (TaKaRa, Japan, RR420A), and gene-specific forward/reverse primers using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher, USA). ..

    Article Title: Generation of spinal cord organoids from human induced pluripotent stem cells caudalised to a lumbar fate.
    Article Snippet: Real-time qPCR for spinal cord region specification Five day 40 organoids were pooled per sample from each differentiation (3 independent differentiations) and RNA was extracted using the RNeasy Mini kit (Qiagen 74104) and RNaseFree DNase set (QIAGEN 79254) as per manufacturer’s protocol. .. For RT-PCR, cDNA was reverse transcribed from 500ng of RNA using the iScript cDNA Synthesis Kit (Bio-Rad 1708891). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) on a CFX96 machine (Bio-Rad). ..

    Article Title: Chronic early-life exposure to diisopentyl phthalate, a plasticizer induced aging and impaired innate immunity via insulin-like and p38 MAPK pathways in Caenorhabditis elegans.
    Article Snippet: Plasticizers are widely used industrial chemicals, in addition, their extensive application has raised growing concerns about potential exposure-related health risks.. Diisopentyl phthalate (DiPP), commonly used in the production of plastics and coatings, remains poorly characterized with respect to its toxicological effects.. In this study, Caenorhabditis elegans was employed as the research model to evaluate the impact of early long-time exposure to DiPP on aging and innate immunity.

    SYBR Green Assay:

    Article Title: Cobinamide, a Vitamin B12 Analog, Attenuates Benzo[a]pyrene and Pyrene Toxicity Through Selective Redox Modulation
    Article Snippet: Band intensity was determined by densitometric scanning using a Li-Cor Odyssey instrument over a range where band intensity was linear. .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific). .. Total RNA was isolated using Trizol reagent (catalog #TR 118; Molecular Research Center, Cincinnati, OH, USA), reverse-transcribed using iScript cDNA synthesis kit (catalog #1708891; Bio-Rad), and PCR was performed using a MX3005P real-time PCR detection system (Agilent Technologies, Inc., Santa Clara, CA, USA) with Brilliant II SYBR Green Mix (catalog #A25742; Thermo Fisher Scientific).

    Article Title: Progranulin deficiency in the brain activates an insulin signaling pathway that may promote neurodegeneration
    Article Snippet: Gene expression was analyzed by real-time quantitative PCR (qPCR) of total RNA isolated from flash frozen tissues with the RNeasy Mini kit (Qiagen, 74106) using on-column DNase digestion (Qiagen, 79256). .. RNA was reverse transcribed to obtain cDNA using the iScript cDNA synthesis kit (Bio-Rad, 1708891), and qPCR was performed using PowerUp SYBR Green Master Mix (ThermoFisher, A25777 ) with a Bio-Rad CFX384 Real-Time System. ..

    Article Title: Generation of spinal cord organoids from human induced pluripotent stem cells caudalised to a lumbar fate.
    Article Snippet: Real-time qPCR for spinal cord region specification Five day 40 organoids were pooled per sample from each differentiation (3 independent differentiations) and RNA was extracted using the RNeasy Mini kit (Qiagen 74104) and RNaseFree DNase set (QIAGEN 79254) as per manufacturer’s protocol. .. For RT-PCR, cDNA was reverse transcribed from 500ng of RNA using the iScript cDNA Synthesis Kit (Bio-Rad 1708891). qPCR was performed using SsoAdvanced Universal SYBR Green Supermix (Bio-Rad 1725271) on a CFX96 machine (Bio-Rad). ..

    Article Title: Chronic early-life exposure to diisopentyl phthalate, a plasticizer induced aging and impaired innate immunity via insulin-like and p38 MAPK pathways in Caenorhabditis elegans.
    Article Snippet: Plasticizers are widely used industrial chemicals, in addition, their extensive application has raised growing concerns about potential exposure-related health risks.. Diisopentyl phthalate (DiPP), commonly used in the production of plastics and coatings, remains poorly characterized with respect to its toxicological effects.. In this study, Caenorhabditis elegans was employed as the research model to evaluate the impact of early long-time exposure to DiPP on aging and innate immunity.

    Quantitative RT-PCR:

    Article Title: Biomimetic hybrid nanoparticles for dual regulation of macrophage pyroptosis and metabolism in rheumatoid arthritis.
    Article Snippet: The concentration and purity of total RNA were determined using a Nanodrop 2000 spectrophotometer (Thermo Fisher, USA). .. A total of 2 μg of qualified total RNA was reverse-transcribed into cDNA using an iScript cDNA Synthesis Kit (Bio-Rad, USA, 1708891). qRT-PCR was performed in a reaction system containing cDNA, SYBR Premix Ex Taq (TaKaRa, Japan, RR420A), and gene-specific forward/reverse primers using a QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher, USA). ..

    Article Title: Selection of appropriate reference genes in Apoe -/- mouse brain
    Article Snippet: The concentrations and purity of RNA samples were measured using a NanoDropTM 2000/2000c Spectrophotometer (ThermoScientific). .. iScript cDNA synthesis kit was used to synthesize cDNA (1708891, BioRad, Hercules, CA, USA) and 500 ng DNase-treated RNA was used for reverse transcription. cDNA samples were diluted 4-fold, and 2 μl of the diluted cDNA was used for RT-qPCR experiments. .. LightCycler 480 SYBR Green I Master mix solution (04887352001, Roche, Mannheim, Germany) was utilized according to the manufacturer‘s instructions.

    Generated:

    Article Title: GIPR:GCGR co-agonism restores normal weight in obese rodents
    Article Snippet: RNA from the liver lysate was extracted using RNeasy mini kit (Qiagen, Cat #74106). .. RNA was extracted using RNeasy lipid tissue mini kit (Qiagen, Cat #74804) or RNeasy Fibrous tissue mini kit (Qiagen, Cat #74704) in the case of muscle. cDNA was generated using iScript cDNA synthesis kit (Bio-Rad, Cat #1708891). .. Gene expression was performed with TaqMan probes and TaqMan gene expression master mix (Cat #4370074) from Applied Biosystems using QuantStudio 5 realtime PCR system (Applied Biosystems).



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